Quantitative Aspects of the Reaction between Insulin

نویسندگان

  • SOLOMON A. BERSON
  • ROSALYN S. YALOW
چکیده

The presence of insulin-binding antibodies in the serums of insulin-treated subjects has been reported previously (1). With I"ll-labeled insulin it has been shown that the antigen-antibody complexes do not precipitate, that the binding of insulin to antibody is a reversible process, and that, at constant antibody concentration, the ratio of bound insulin to free insulin is an inverse function of the concentration of insulin (1). In the present studies, experimental data on the reaction between insulin and insulin-binding antibody are tested against several theoretical models. The results are most compatible with a model composed of a univalent insulin reacting with two distinctly different orders of antibody sites. Data for equilibrium constants, forward and reverse velocity constants and various thermodynamic constants are presented in terms of the model chosen.

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تاریخ انتشار 2013